Effects of manipulating expression of acetyl-CoA carboxylase I in Brassica napus L. embryos.
نویسندگان
چکیده
Acetyl-CoA carboxylase I (ACCase I) in developing oilseed rape embryos is predominantly cystosolic, based upon measurement of its propionyl-CoA carboxylase activity. Reduction of ACCase I by antisense expression reduces seed lipid content and affects carbohydrate metabolism.
منابع مشابه
Biotin carboxyl carrier protein and carboxyltransferase subunits of the multi-subunit form of acetyl-CoA carboxylase from Brassica napus: cloning and analysis of expression during oilseed rape embryogenesis.
In the oilseed rape Brassica napus there are two forms of acetyl-CoA carboxylase (ACCase). As in other dicotyledonous plants there is a type I ACCase, the single polypeptide 220 kDa form, and a type II multi-subunit complex analogous to that of Escherichia coli and Anabaena. This paper describes the cloning and characterization of a plant biotin carboxyl carrier protein (BCCP) from the type II ...
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One independent and two overlapping rape cDNA clones have been isolated from a rape embryo library. We have shown that they encode a 2.3 kb and a 2.5 kb stretch of the full-length acetyl-CoA carboxylase (ACCase) cDNA, corresponding to the biotin-binding and transcarboxylase domains respectively. Using the cDNA in Northern-blot analysis we have shown that the mRNA for ACCase has a higher level o...
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ورودعنوان ژورنال:
- Biochemical Society transactions
دوره 28 6 شماره
صفحات -
تاریخ انتشار 2000